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Image Search Results
Journal: STAR Protocols
Article Title: Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging
doi: 10.1016/j.xpro.2022.101828
Figure Lengend Snippet: Sample preparation and staining panel—Mouse HSC
Article Snippet:
Techniques: Sample Prep, Staining, Positive Control
Journal: STAR Protocols
Article Title: Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging
doi: 10.1016/j.xpro.2022.101828
Figure Lengend Snippet: FACS gating strategy to sort MMP-Low or -High CD48-LSK CD150 + mouse HSCs (A) Determine Lin - CD48 - gate (Gate 1). Flow cytometry APCCy7 contour plots of total BM (Lin/CD48)-APCCy7 positive staining control (i), and Lin - enriched BM full stain sample (ii). (B) Flow cytometry gating scheme towards CD48 - LSKCD150 + from Lin - enriched mouse BM. i, ii, ii, iv, gating debris - (i), doublets - (ii, iii), DAPI - (iv) single viable cells. v, vi, vii, sequential gating of Lin - CD48 - BM (Gate 1; v), Sca-1 + c-Kit + (Gate 2;vi), CD150 + (Gate 3; vii). viii, TMRE Flow histogram of CD48 - LSKCD150 + HSCs. Set bottom 25% MMP-Low and top 25% MMP-High gates.
Article Snippet:
Techniques: Flow Cytometry, Staining
Journal: STAR Protocols
Article Title: Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging
doi: 10.1016/j.xpro.2022.101828
Figure Lengend Snippet: Validation of the specificity and responsiveness of TMRE staining to changes in MMP level using CCCP and oligomycin Flow cytometry histogram of TMRE for CD48 - LSKCD150 + HSCs treated with CCCP (100 μM, red), or oligomycin (2 μg/mL, blue), or untreated (DMSO, 10 min, black). Depolarized and hyperpolarized TMRE gates were determined according to the peak separation on TMRE profile of CCCP and oligomycin treated samples respectively. Only 0.96% or 1.44% untreated HSCs are depolarized or hyperpolarized. TMRE intensity of 97.6% of cells are within physiological range.
Article Snippet:
Techniques: Staining, Flow Cytometry
Journal: STAR Protocols
Article Title: Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging
doi: 10.1016/j.xpro.2022.101828
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Cell Isolation, Selection, Software